Biotechnology/Biomanufacturing (BTC)

Courses

BTC 101. Introduction To Biotechnology I. 4 Credits.

Topics in this course are designed to acquaint students with the diverse field of biotechnology and to develop fundamental skills in the use, maintenance and calibration of common laboratory instruments. Principles of laboratory safety, documentation and the use of computers in the lab will be covered. Topics will also include a brief history of biotechnology, job opportunities in biotechnology, biology of DNA, recombinant DNA and protein products. Lab sessions will be hands-on experiences revolving around and applying the topics listed in the lab section of the syllabus. Prerequisites: ENG 098, FYE 101, MAT 096 or higher (or corequisite), RDG 098, or placement. Fall.

BTC 102. Introduction to Biotechnology II. 4 Credits.

Topics in this course are designed to acquaint students with the diverse field of biotechnology and to develop fundamental skills in the set-up of typical growth plates and media used in standard culturing of microorganisms or tissue culture, as well as preparation of common solutions and reagents. Topics will also include microbial biotechnology, plant biotechnology, medical biotechnology, DNA fingerprinting and forensic analysis. Current ethical issues, such as stem cell research and cloning, will also be discussed. Lab sessions will be hands-on experiences revolving around and applying the topics listed in the lab section of the syllabus. Prerequisites: ENG 098, FYE 101, MAT 096 or higher (or corequisite), RDG 098, or placement.

BTC 110. Biotechnology Experience: Concepts And Procedures. 4 Credits.

The first of two experiential, cornerstone courses in biotechnology. The course begins by introducing the student to the role of the technician in biotechnology and GLP (or Good Laboratory Principles). An exploration of the principles of molecular genetics will be provided, including lecture topics in transcription, translation and protein expression in prokaryotes, and eukaryotes. Included in the course will be a hands-on exposure to biotechnology research tools and protocols used for DNA isolation, gene cloning, gene expression and regulation, gene sequencing, and mRNA isolation. Lab sessions will be hands on experiences revolving around and applying the topics listed in the lab section of the syllabus. Prerequisites: BTC 101, BTC 102 with a C or better.

BTC 120. Biotechnology Experience: Biomanufacturing. 4 Credits.

The second of two experiential courses, this course begins by introducing the student to the proteins and companies of biotechnology. In the remainder of the course students use bacteria, mammalian, and yeast cells to produce human proteins using tools and standard manufacturing operating procedures of biotechnology, including upstream and downstream processing of proteins, and quality control of protein production. Lab sessions will be hands-on experiences revolving around and applying the topics listed in the lab section of the syllabus. Prerequisites: BTC 101 with a grade of C or better, BTC 102 (or corequisite). Spring.

BTC 170. Cell Culture Techniques. 4 Credits.

This lecture/laboratory course will provide students with a solid understanding of basic sterile and aseptic cell culture techniques through the growth and maintenance of both normal and transformed adherent and suspension mammalian cells. Topics include sterile culture techniques, trypsinization and media supplementation, subculturing, cell counting, determining viability and growth curves, cryopreservation, and research applications using cell cultures. Prerequisite: BIO 109; BTC 101 and BTC 102 with a C or better. Spring.

BTC 185. Industrial Microbiology. 4 Credits.

This course covers the principles of various processes associated with the production and recovery of different bioproducts derived from prokaryotes and eukaryotes. Topics include the classification of microorganisms, media development, instrumentation, fermentation principles, mammalian and insect cell propagation, product recovery, protein purification, and the principles of current good manufacturing practices (cGMP). Lab sessions will be hands on experiences revolving around and applying the topics listed in the lab section of the syllabus. Prerequisite: BTC 101 with a C or better OR AQS 110, and BTC 102 with a C or better.

BTC 190. Principles of Protein Purification. 4 Credits.

The biopharmaceutical industry is focused on producing proteins as drugs to treat diseases. This course will investigate the relationship between protein structure and function, present basic methods of protein purification, and demonstrate how protein composition influences the method of purification. The concept of pH and buffers will be discussed in detail in relation to protein purification. Students will be exposed to these concepts in lecture, readings, and the lab. Lab sessions will be hands on experiences revolving around and applying the topics listed in the lab section of the syllabus. Prerequisites: BTC 101, BTC 102 (or corequisite), CHE 107.

BTC 191. Quality and Compliance. 3 Credits.

Good Manufacturing Practice regulations (cGMPs) apply to pharmaceutical, biotech and medical device products regulated by FDA. This course explores the content and interpretation of the regulations, with emphasis on the necessary practices, procedures and documentation required to achieve full compliance. The course will utilize case studies to facilitate interpretation and application of regulations. All participants will gain a fundamental knowledge of the basis of cGMP regulations, and the necessity of implementing them in daily operations. Additionally, this course is intended to give participants an introduction to the cGMP regulations and their application to laboratory activities, manufacturing processes and support functions, as well as demonstrate the need for thorough and comprehensive GMP training and documentation. The course also provides participants with an understanding of common terminology and the role GMPs play in their day-to-day responsibilities. Consequences of non-compliance will be examined to underline the importance of implementing and maintaining a robust cGMP posture. Prerequisites: BTC 101 with a C or better OR AQS 110 and BTC 102 with a C or better.

BTC 200. Biotechnology Seminar. 3 Credits.

This is a capstone course taken in the last semester before graduation after all of the BTC courses have been successfully completed. This course will provide an in-depth review of the entire process of producing a biologic drug. Students will examine current research being conducted in the biotech industry and explore cGMP regulations through examination of FDA warning letters. As a capstone course, students will be expected to demonstrate mastery and integration of previous BTC major coursework. Prerequisites: BTC 101 and BTC 102 with a C or better; BTC 120, BTC 170(or co-requisite). Spring.